Regulatory

Part:BBa_K2570015:Design

Designed by: Xueyi Yang   Group: iGEM18_FJNU-China   (2018-09-30)


dsrA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dsrA is a temperature control promoter. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher.


Source

E.coli

References

[1] Temperature Sensing by the dsrA Promoter. JOURNAL OF BACTERIOLOGY, Nov. 2003, p. 6609–6614 DOI: 10.1128/JB.185.22.6609–6614.2003

[2] Sledjeski, D. D., A. Gupta, and S. Gottesman. 1996. The small RNA, DsrA, is essential for the low temperature expression of RpoS during exponential growth in Escherichia coli. EMBO J. 15:3993–4000

[3] Majdalani, N., C. Cunning, D. Sledjeski, T. Elliott, and S. Gottesman. 1998. DsrA RNA regulates translation of RpoS message by an anti-antisense mechanism, independent of its action as an antisilencer of transcription. Proc. Natl. Acad. Sci. USA 95:12462–12467.

[4] Repoila, F., and S. Gottesman. 2001. Signal transduction cascade for regulation of RpoS: temperature regulation of dsrA. J. Bacteriol. 183:4012–4023.